AIM: To learn the technique of Counter Current Immunoelectrophoresis. PRINCIPLE: Immunoelectrophoresis is the electrophoresis of a determined antigen. A counter current electrophoresis technique for the rapid detection of Mycoplasma mycoides subsp, Mycoides antigen in tissue from animals infected with. Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an See also. Immunoelectrophoresis · Electrophoresis protein · Procalcitonin · Total complement activity · MELISA · Complete blood count · lymphocyte count.
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Bacteriological findings in pneumonia.
J Hyg Lond Jun; 53 2: Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type J Lab Clin Med. Non-tuberculous bacterial flora of sputum and of the upper and lower respiratory tract in pulmonary tuberculosis. Immunologic investigations of meningococcal disease. N Immuboelectrophoresis J Med. Immunoprecipitation Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis.
Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen immuhoelectrophoresis, it is similar to immunodiffusionbut counger the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel.
The clinical importance of these findings is still being studied but our experience has shown that patients with chest infections should have effective antipneumococcal antibiotics as part immunoeleftrophoresis their regimen and that the laboratory diagnosis may be made quickly and accurately by counter-current immunoelectrophoresis.
The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a precipitin line, indicating binding. Associated Data Supplementary Materials.
Counterimmunoelectrophoresis – Wikipedia
Diagnostic immunology Nephelometry Complement fixation test Immunocytochemistry Immunohistochemistry Direct fluorescent antibody Epitope mapping Skin allergy test Patch test.
Pneumococcal typing and the problem of endogenous or exogenous reinfection in chronic bronchitis. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. National Center for Biotechnology InformationU.
Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.
Group-specific Neisseria meningitidis antigens present in the serum of patients with fulminant meningococcemia. The chemotherapy of bacterial respiratory infections. Open in a separate window. Bacteriologic flora of the lower respiratory tract.
The optimum conditions for performing the test are given. Abstract Counter-current immunoelectrohporesis is a rapid sensitive method for detecting counterr capsular antigens in sputum. Retrieved from ” https: M El-Refaie and C Dulake. Better precipitin lines are obtained when the gel-support is acid pH than when it is alkaline pH Types of pneumococci found in blood, spinal fluid and pleural exudate during a period of 15 years Nosocomial respiratory infections with gram-negative bacilli.
Acta Pathol Microbiol Scand. Am Rev Respir Dis. Br J Dis Chest. From Wikipedia, the free encyclopedia.
Value of dilute inocula in cultural examination of sputum. Pneumococcal antigen in lobar pneumonia. Pneumococcal antigen in sputum.