Euphorbia hirta has been widely used by Tribal as traditional medicine in a treatment against infectious pathogens. E. hirtaLinn. is a perennial. diffusion method was used to determine the antibacterial activity on gram negative: Euphorbia hirta plant displayed an excellent antibacterial activity against K. The antibacterial and antifungal effect of Euphorbia hirta was evaluated against a panel of bacteria (Escherichia coli, Psueudomonas aeruginosa, Klebsiella.

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Bacterial and fungal suspensions were used as negative control, while broth containing standard drug was used as positive control. In all test microbes most susceptible organism in the investigation was C.

Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Table 3 Antifungal activity of flavonoids of Euphorbia hirta by disc diffusion assay.

The euphorbi was carried out along with standard drugs terbinafine and itraconazol for fungus and streptomycin for bacteria. In the current investigation E. Antibacterial activity and toxicological potentials eupyorbia crude ethanolic extracts of E.

Out of the four bacteria tested, S. Free and bound flavonoid extract exhibited good inhibitory activity against most of the pathogens. Different parts root, stem, leaf, and fruits of E. Received Jul 5; Accepted Sep 9. Preparative TLC of the bound flavonoids from stem of E. In the present study, total eight extracts of different parts of plants were tested for their bioactivity. Results of the present study reveals that all the eight tested plant extracts inhibit the growth of selected bacteria and fungi; indicating broad spectrum bioactive nature of selected plant.


Results of the present investigation indicate that E. Free and bound flavonoids from root, stem leaf, and fruits of E. Each dilution was added to broth media of wells of microtiter plates.

Antimicrobial activity of essential oil extracts of various onions Allium cepa and garlic Allium sativum Lebensm-Wiss-U-Technol. Acttivity information Article notes Copyright and License information Disclaimer. Table 1 Rf values of spots of flavonoid extract obtained in TLC solvent system benzene: Such studies could only indicate their antimicrobial potential, but are not helpful in establishing them as an antibiotic.

Antibacterial and antifungal activity was expressed in terms of activity index AI. The medicinal plants of Mauritius. Continued further exploration of plant-derived antimicrobials is need of the day.

TA determination TA is the volume at which the test extract can be diluted with the ability to kill the microorganisms. The present investigation evaluates the antibacterial and antifungal effects of free and bound flavonoids of E. Open in a separate window.

Antimicrobial potency of flavonoids free and bound of E. Wong E, Francis CM. Full TA calculated against E.

New antimicrobials of plant origin. The potential for developing antimicrobials from higher plants appears rewarding as it will lead to the development of phytomedicine to act against microbes. Eight pathogenic microorganisms in total, including four bacteria, viz. Larvicidal activity of some Euphorbiaceae euphorvia extracts against Aedes aegypti and Culex quinquefasciatus Diptera: Traditional medicine practice amongst the igede people of Nigeria.


The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Elutes were pooled, completely dried, and rechromatographed to test the purity of the isolated compound.

Petroleum ether fractions were discarded as being rich in fatty substances, whereas ethyl ether and ethyl acetate fractions were analyzed for free and bound flavonoids, respectively. Antimiccrobial and quantity of extracts from plant parts was calculated and recorded [ Table 6 ].

Geeta Singh and Padma Kumar.

Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta

There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action for new and reemerging infectious diseases. MIC was determined for the plant extract showing antlmicrobial activity against test pathogens. Assessment of bioactivity of Indian medicinal plants using Brine Shrimp Artemia salina lethality assay.

Broth microdilution method was followed for determination of MIC values. Financial assistance provided by UGC is gratefully acknowledged.